The SPACEHAB single module carried nearly 1,400 kilograms (3,100 lb) of experiments and support equipment for 12 commercial space product development payloads in the areas of biotechnology, electronic materials, polymers and agriculture as well as several experiments for other NASA payload organizations. One of these, the Commercial Float Zone Facility (CFZF) was developed through international collaboration between the U.S., Canada, and Germany. It heated various samples of electronic and semiconductor material through the float-zone technique. Another facility on SPACEHAB was the Space Experiment Facility (SEF) which grew crystals by vapor diffusion.

The Goddard Space Flight Center's (GSFC) Spartan-207 satellite was used to deploy and test the Inflatable Antenna Experiment (IAE) which laid the groundwork for future technology development in inflatable space structures. It tested the performance of a large inflatable antenna during a ninety-minute mission. The antenna structure was then jettisoned and the SPARTAN-207 spacecraft recovered at mission end.

Pilot Curtis Brown prepares to activate the Fluids Generic Bioprocessing Apparatus (FGBA) 2, on the middeck.
Inside Endeavour's cargo bay the four TEAMS experiments operated throughout the mission. They included the Global Positioning System (GPS) Attitude and Navigation Experiment (GANE) to determine to what accuracy the GPS system can supply attitude information to a space vehicle; the Vented Tank Resupply Experiment (VTRE) to test improved methods for in-space refueling; the Liquid Metal Thermal Experiment (LMTE) to evaluate the performance of liquid metal heat pipes in microgravity conditions, and the Passive Aerodynamically Stabilized Magnetically Damped Satellite (PAMS) payload to demonstrate the technology of the principle of aerodynamic stabilization in the upper atmosphere. Cameras on the shuttle recorded the PAMS satellite as it was deployed and tracked its movements.

Secondary experiments on the flight included the Brilliant Eyes Ten Kelvin Sorption Cryocooler Experiment (BETSCE), the Aquatic Research Facility (ARF) and the Biological Research In a Canister (BRIC) experiment.

Also onboard was the Plant-Generic Bioprocessing Apparatus (P-GBA) designed by BioServe Space Technologies. Several plant species were flown in this double middeck locker configurated plant growth chamber. Investigations on plant growth in micro-gravity as well as research on the feasibility of agriculture in space were successfully carried out.

A Coca-Cola fountain dispenser (officially a Fluids Generic Bioprocessing Apparatus-2 or FGBA-2) was developed for use on STS-77 as a test bed to determine if carbonated beverages can be produced from separately stored carbon dioxide, water and flavored syrups and determine if the resulting fluids can be made available for consumption without bubble nucleation and resulting foam formation. The unit held 1.65 liters each of Coca-Cola and Diet Coke. The original FGBA flew on STS-63.