Command Pilot: Daniel C. Brandenstein (Third Space Flight)
Pilot: James D. Wetherbee (First Space Flight)
Mission Specialist 1: Bonnie J. Dunbar (Second Space Flight)
Mission Specialist 2: G. David Low (Second Space Flight)
Mission Specialist 3: Marsha S. Ivins (First Space Flight)

STS-32 was the 33rd mission of NASA Space Shuttle programme, and the 9th launch of Space Shuttle Columbia. Launched on 9 January 1990, it marked the first use of Launch Pad A at Kennedy Space Centres Complex 39 since 1986; it also marked the first use of MobileLauncher Platform No. 3 (MLP-3) in the Space Shuttle programme. STS-32 was, at the time, the longest shuttle mission yet conducted, with a duration of nearly 11 days. Before STS-32, the only mission of the same duration had been STS-9 in 1983. On 20 January 1990, STS-32 executed the third night landing of the shuttle programme.